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Given the independent replication of our observed directionality in an external cohort using EHR data, the directionality inferred by our study is unlikely to be the result of a data quality issue
Briefly, 1 mL of assay mixture contains optimized concentrations of the following chemicals: 0,5 M K 2 HPO 4 (pH 7.0), 2,5 mM EDTA, 0,18 U/mL GR, 100 mM glutathione and 10 mM reduced NADPH and tissue extract (0,5 mL) was added in the spectrophotometer cuvette along with 0,1 mL of 60 mM cumene hydroperoxide, a suitable substrate for GP
"Reaction mechanism of glutathione synthetase from Arabidopsis thaliana: site-directed mutagenesis of active site residues"
Detoxifies the skin
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