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published online: 19 January 2017
The annealed DNA was then serially mixed in a 3:1 molar ratio with concentrated Pol catalytic domain (Val235-Trp575, 12.74 mg/mL)followed by addition of the incoming nonhydrolyzable dUMPNPP nucleotide (0.91 mM final concentration)
Side effects are uncommon and usually mild: Soreness or redness at the injection site Mild digestive upset Allergic reactions, which are rare Because a shot is an injection, it should always be given by a trained provider
Serious adverse events are rare but documented
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