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Subsequently, LH slices were incubated in guinea-pig anti-cFos (1:1000, Synaptic Systems) diluted in [PBS 0.01 M + 0.3% Triton + 10 mM Glycine + 0.1% H 2 O 2 ]
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Dosages are often empirically determined, and the potential risks of overdosing, including the onset of RS, are seldom considered [52] ( Table 2 )
However, they should try to take each dose at roughly the same time of day and close to meal times
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