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17), no se sabe si una ingesta diettica de colina subptima en sujetos sanos puede contribuir a un mayor riesgo de NAFLD
Protein-lipid overlay assay After 24 h of transfection, N2a cells were washed twice with TBS (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 10% glycerol) and cell extracts were prepared as explained above in TBS supplemented with 0.5% Tx-100 and protease inhibitors
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