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Acknowledgements We thank Celina Nhan, Jack Chen, Nina Juntereal, Anh Cao, Claudia Liberini, and Samantha Fortin for valuable technical assistance
With a half-life of about six days, it takes around four to five half-lives to clear, during which its appetite-regulating and metabolic effects gradually diminish
LR3s 20-30 hour half-life provides systemic support for overall mass
Thermo Fisher Scientific, Inc.) and transferred to Invitrolon polyvinylidene difluoride membranes (Thermo Fisher Scientific, Inc.), which were subsequently blocked for 1 h with 5% bovine serum albumin (Thermo Fisher Scientific, Inc.) in Tris-buffered saline supplemented with Tween-20 (Cell Signaling Technology, Inc.)
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