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Thiol groups are strong reducing agents thus glutathione serves as an electron donor and in the process is converted to its oxidised form

Check the Certificate of Analysis (COA) for: Identity confirmation (MS data matching expected molecular weight) HPLC purity (greater than 98% is recommended for reliable research work) Fill weight (the actual mass of lyophilized peptide in the vial, which may differ slightly from the nominal amount) Expiration or retest date Using the fill weight from the COA rather than assuming the labeled amount ensures your concentration calculations are accurate

This determines the final concentration of the solution