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The section was then incubated with recombinant anti-mucin 5AC antibody (ab3649, Abcam) for 1 h at room temperature, followed by biotinylated secondary antibody (Dako) and HRP detection (HRP conjugated ABC system, Vector Laboratories), according to manufacturers instruction
The Recommended Dietary Allowance (RDA) for magnesium is 400-420 mg daily for adult men and 310-320 mg for adult women
However, the limited follow-up duration of 6 months raised concerns about potential reverse causality, as undetected suicidal ideation and medical conditions might have influenced the choice of particular anti-obesity or anti-diabetic drugs
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