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rAA is measured in one aliquot of a sample and total AA (that is, rAA and DHA) in a second aliquot of the same sample in which the DHA is reduced by incubation with tris(2-carboxyethyl) phosphine hydrochloride (TCEP, 0.5 mM) in the dark for 30 min at room temperature
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Breath Rate, BR
Cell Viability Assay The cell viabilities of different concentrations of GHK, Cu(OAc) 2 and CuCl 2 against Human adult low Calcium high Temperature (HaCaT) keratinocytes were studied by MTT assay in 6 replicates
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