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Shankar, V
Readouts most often include glycerol release and free fatty acid quantification in the supernatant, alongside cellular ATP or viability measures to confirm that observed lipolysis reflects genuine metabolic signaling rather than cytotoxic membrane disruption
Glutathione colorimetric assay kit is designed to measure reduced glutathione (GSH), oxidized glutathione (GSSG) and total glutathione (GSH+GSSG) concentrations in wide range of samples like, blood, plasma, serum, cultured cells and tissue
The infection of cell monolayers was performed using a multiplicity of infection (MOI) of 30:1 (9 10 6 bacteria: 3 10 5 cells)
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