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Reaction mixtures (3.0 mL) contained 2.5 mL 0.1 M sodium phosphate buffer (pH 6.5), 0.1 mL crude enzyme extracts, 0.1 mL peroxidase (250 UmL 1 ), and 0.2 mL 4-aminoantipyrine/N, N-dimethylaniline
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