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Ultrastructural analysis by the glycocalyx method revealed that detachment of the intrahepatic microvessels in the MCD group compared to the control group

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the pool containing Arg 34 GLP-1 (7-37) and various impurities was diluted with three volumes of water, and 40 ml of the resulting solution was applied to a 20 ml Source 15Q (Amersham Pharmacia Biotech) column equilibrated with 100 ml 20 mM Tris-hydroxymethyl amino-methane, pH 8.5