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In addition to a medium made of 0.5 ml of DMEM, 10% FBS, 100 U/mL of penicillin, and 100 mg/mL of streptomycin, BPC 157 was added to each well in concentrations of 0 (control group), 0.1, 0.25, and 0.5 ug/mL for 1, 2, and 3 days in a humidified atmosphere
DSIPs mechanism of action in the CNS appears to warrant intranasal application as a non-invasive means to directly cross the BBB
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FDA inspections have repeatedly found research-grade peptides with inaccurate dosing and contamination, which is actually why the FDA moved several peptides to Category 2 back in 2023
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