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After purification, islets were cultured overnight at 37 C in a humidified atmosphere of 95% air and 5% CO 2 in RPMI 1640 medium supplemented with 10% FBS, 100 units/ml penicillin and 0.1 mg/ml streptomycin

10.1016/j.arth.2025.09.054 29 LiH.GeorgeD

Numerous evidences indicate that obesity leads to systemic chronic inflammation and is strongly associated with type 2 diabetes, cardiovascular disease, non-alcoholic fatty liver disease, along with many types of cancers (Rohm et al., 2022)

Their role in the pathophysiology of type 2 diabetes (T2D) has been well-characterized, and augmentation by incretin-based therapies leads to improved glycemic control 6