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The luciferase assay was performed with a dual-luciferase reporter assay system (Promega, USA)
Telomere length as a marker of oxidative stress in primary human fibroblast cultures
PDEs activity For kinetic analysis of PDEs activity, the PDEs, 3,5-nucleotides, and cAMP (Abcam plc, Cambridge, UK), were used at concentrations ranging from 0.3 M to 40 M of cAMP and the reaction interval was 30 min according to the instrumental procedure
It is a tripeptide, meaning it is composed of three amino acids: glycine, histidine, and lysine
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