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Digitonin fractionation Cells were lysed for 30 min on ice with extraction buffer (250 mM sucrose

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Hypothesis 3: Storage temperature will significantly impact levels of total GSH, free GSH, GSSG, and the ratio of free GSH to GSSG

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Elevated IDO/TDO activity, often assessed through the KYN/TRP ratio, leads to increased TRP metabolism via the KP at the expense of 5-HT synthesis

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