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Intestinal microbiota analysis Total genomic DNA was extracted from ileal content samples (n = 5 per group) using a TGuide S96 Magnetic Soil/Stool DNA Kit (Tiangen Biotech (Beijing) Co., Ltd.) according to the manufacturers instructions

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Lipoprotein 2 significantly promotes ERS (upregulation of GRP78 and NOGO transcription, increased expression of SOD2, and slightly enhanced mitochondrial membrane potential) and proliferation (as evaluated by Ki67 staining and BrdU incorporation) in PH (pulmonary hypertension) while simultaneously increasing intracellular iron levels in human PASMC cells

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