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MTX-induced apoptosis was significantly attenuated with all treatments except for NTZ-100, where, as depicted in Figures 5AD, Bcl-2 level was augmented while Bax was reduced, and consequently, Bax/Bcl-2 ratio was also reduced as well as caspase-3 activity all when compared to MTX group

Off-label use at lower doses (such as 1 mg daily) is explored in wellness contexts but lacks equivalent RCT support at those doses

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Thus the GSH/GSSG ratio is important to normal functioning and is a likely contributor to cellular signaling pathways by activating or inactivating key thiol-containing enzymes