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A small amount of urine is collected and tested using a urine test strip

We thus performed OGTTs on Glp1r OB-hM3Dq WD mice after chemogenetic activation of OB Glp1r -expressing neurons with concurrent blockade of GABA A R in the PVN, using a local injection of the GABA A R antagonist bicuculline (Fig

Avoid "fanning" techniques whenever possible, which involve multiple injections in a fan-like pattern