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Atvb 25, 19451951
Stacks Well With: What Can I Expect
The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM
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