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Functionally, NSPCs isolated from Sprague-Dawley rats, and treated with 10 nM E2 for 2 days, significantly increase their proliferative rate, while doses of 50 nM notably inhibit this process

Severe Adverse Reactions to High-Dose Vitamin B12 Severe reactions to high-dose vitamin B12 are rare but can be deadly

HEK293T cells were cultured as previously described

Proteins were analyzed by immunoblotting using the following primary antibodies: anti-Gstt1 (Abcam, ab175418), -actin (Sigma-Aldrich, A5316), Tubulin (Sigma-Aldrich, T6199), E-Cadherin (BD, 610181), Vimentin (BD, 550513), PCNA (PC10) (Santa Cruz, sc-56), Fibronectin (Abcam, ab199056), GSH (D8) (Santa Cruz, 52399)