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Bacteria were washed off the agar with 2 mL M9 buffer, subjected to OD 600 measurement using a DS-11 FX+ spectrophotometer (DeNovix), and bacterial density determined by serial dilution, plating on agar plates, and counting colony-forming units (CFU) 64

The study also highlighted AOD9604s influence on fat metabolism and energy expenditure, and its interaction with 3-adrenergic pathways [2]

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