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To test protease inhibitors, pseudotyped viruses were preincubated with medium (DMEM with 7.5% FBS and 50 g/mL elastase) containing the indicated concentrations of each protease inhibitor (Nafamostat Mesylate: Cat# N0959, Tokyo Chemical Industry Co., Ltd., Tokyo, Japan, Decanoyl-RVKR-CMK: Cat# 3501, Tocris Bioscience, Bristol, U.K) at room temperature for 5 min and then added to the wells
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