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Thus, researchers have employed X-ray crystallography to ascertain the crystallographic structures of the extracellular domains of TLRs and their respective ligand complexes
When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature
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