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Collagen is a structural protein primarily focused on replenishing the structural framework of the skin, leading to improved elasticity and reduction of deep wrinkles
Following the manufacturers instructions, quantitative PCR was carried out using an ABI Prism StepOnePlus Real-Time PCR System (Applied Biosystems) and PowerTrackTM SYBR Green Master Mix Applied BiosystemsTM 45,46
Patients should not attempt to accelerate dose increases independently, as this significantly raises the risk of adverse effects including nausea, vomiting, and gastrointestinal discomfort
These are not guaranteed outcomes for every patient, but they are consistent with the broader health improvements typically associated with clinically significant weight loss
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