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doi:10.1021/acs.jmedchem.1c00565 Dehestani B, Stratford NR, le Roux CW
Increase water intake, add fiber gradually (not all at once), and consider magnesium citrate as a gentle osmotic support
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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution
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