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In the 2D-DIGE analysis, proteins for a total of 361 spots were identified by MALDI-TOFMS analysis
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Complementary approaches such as siRNA-mediated NNMT knockdown, shRNA-mediated knockdown, CRISPR-based perturbation, or comparison with a structurally distinct NNMT inhibitor can help determine whether an observed phenotype follows NNMT biology rather than an unrelated chemical or solvent-associated effect
Experimental evidence from AD models suggests that excessive BCAA availability may aggravate neuropathology
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