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M., Zhang, Z
Evolving procaspase-1-cleaving proteases with reduced off-target cleavage activity Although X(PC) demonstrated high selectivity for procaspase-1 over VAMP1, its transfection into mammalian cells lacking procaspase-1 (HEK293T cells) still resulted in reduced cell viability compared to a catalytically inactive X(PC) variant, consistent with residual off-target activity (Extended Data Fig
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