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1) Injection Site : Injection site reactions have been reported very rarely
After homogenization, 1 mL of extraction solution (isopropanol: HO: HCl = 2:1:0.002) was added, and the mixture was sonicated for 30 min at 4 C in the dark using a KQ5200DE ultrasonic cleaner (Kunshan Shumei Ultrasonic Instruments, Suzhou, China)
Notably, ILC2 proportions were comparable between IL-33 alone and IL-33 plus melatonin and Luzindole groups ( Figures 2K, L ), yet BALF concentrations of IL-5 ( Figures 2M ) and IL-13 ( Figures 2N ) remained significantly reduced with melatonin treatment, again indicating dampened ILC2 activity rather than altered ILC2 abundance
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