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H 2 can also activate autophagy by inhibiting stress-related p38 and JNK/MAPK pathways (Guan et al., 2019)

Needle gauge matters too: a finer needle (commonly 29-31 gauge for insulin syringes) reduces the mechanical stress placed on the solution as it passes through the needle bore compared to a larger-bore needle, and produces a smaller, cleaner puncture through the vial's rubber stopper, reducing the risk of coring small rubber fragments breaking off into the solution