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That is the proposed reason it does not raise IGF-1 levels or impair insulin sensitivity in animal work, in contrast to full-length growth hormone

Pellets were thawed, cells were lysed with buffer (Beyotime) containing 50 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, supplemented with complete protease inhibitor mixture (Beyotime)

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