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The fluorescence signal was measured at the end of each extension step

15 : 421427 Levine J, Barak Y, Gonzalves M, Szor H, Elizur A, Kofman O et al

Taking too little might not give you the desired results, while too much can sometimes lead to unwanted side effects

Similarly, if I draw now what a nucleus would like in a cell, when our DNA is very young our cells function perfectly, essentially perfectly because the genes that should be on in a particular cell type, lets say the RGCs at the back of the eye in the optic nerve, they know that theyre nerves because the gene for being a nerve is switched on, but unfortunately what we see is that over time these genes get switched off, and vice versa