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Only JY4 and TAK-063 formed hydrogen bonds within the active site, whereas Compound 86 lacked hydrogen bonding, potentially explaining its lower affinity (Figures 27AC)

It emphasizes that mild or transient heat stress primarily activates protective proteostasis-maintaining responses, whereas sustained or severe heat stress may exceed the compensatory capacity of the molecular chaperone system, thus promoting oxidative stress, apoptosis, and impaired spermatogenesis