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intravenous (some studies) Protocol: Cyclic -- 5-10 mg/day for 10-20 consecutive days, repeated every 4-6 months (2-3 cycles per year) Rationale: Brief telomerase activation extends telomeres, which then provide sustained replicative capacity during drug-free intervals Requirements: Sterile reconstitution, cold chain storage, injection supplies, injection technique Regulatory status: FDA Category 2 since 2024 (banned from compounding in the US) NAD+# Route: Oral (NMN, NR supplements)

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The soft tissue injury model was established by mechanical stress